BMRB Entry 6479
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6479
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Title: The Structure of the Excisionase (Xis) Protein from Conjugative Transposon Tn916 Provides Insights into the Regulation of Heterobivalent Tyrosine Recombinases PubMed: 15733914
Deposition date: 2005-02-02 Original release date: 2005-04-04
Authors: Abbani, M.; Iwahara, M.; Clubb, R.
Citation: Abbani, M.; Iwahara, M.; Clubb, R.. "The structure of the excisionase (Xis) protein from conjugative transposon Tn916 provides insights into the regulation of heterobivalent tyrosine recombinases " J. Mol. Biol. 374, 11-25 (2005).
Assembly members:
Excisionase from transposon Tn916, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: Enterococcus faecalis Taxonomy ID: 1351 Superkingdom: Eubacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Excisionase from transposon Tn916: AGHMKQTDIPIWERYTLTIE
EASKYFRIGENKLRRLAEEN
KNANWLIMNGNRIQIKRKQF
EKIIDTLDAI
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 458 |
| 13C chemical shifts | 310 |
| 15N chemical shifts | 77 |
| coupling constants | 32 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Excisionase from transposon Tn916 | 1 |
Entities:
Entity 1, Excisionase from transposon Tn916 70 residues - Formula weight is not available
| 1 | ALA | GLY | HIS | MET | LYS | GLN | THR | ASP | ILE | PRO | |
| 2 | ILE | TRP | GLU | ARG | TYR | THR | LEU | THR | ILE | GLU | |
| 3 | GLU | ALA | SER | LYS | TYR | PHE | ARG | ILE | GLY | GLU | |
| 4 | ASN | LYS | LEU | ARG | ARG | LEU | ALA | GLU | GLU | ASN | |
| 5 | LYS | ASN | ALA | ASN | TRP | LEU | ILE | MET | ASN | GLY | |
| 6 | ASN | ARG | ILE | GLN | ILE | LYS | ARG | LYS | GLN | PHE | |
| 7 | GLU | LYS | ILE | ILE | ASP | THR | LEU | ASP | ALA | ILE |
Samples:
sample_1: Excisionase from transposon Tn916, [U-13C; U-15N], 1 mM; NaOAc 30 mM; NaCl 60 mM; H2O 93%; D2O 7%
sample_cond_1: pH: 4.5 na; temperature: 303 K; ionic strength: 60 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C CT-HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-13C-1H HCCH-COSY | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 13C-1H-1H NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HCACO | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
| 4D 1H-15N-1H-13C NOESY | not available | not available | not available |
Software:
XWINNMR v2.6 - collection
X-PLOR v3.1 - processing
NMRPipe vsgi6x - processing
Pipp v4.3.5 - data analysis
XPLOR-NIH - refinement
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker AMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG12509 BAG80636 BAK28445 GAD37681 |
| EMBL | CAA43359 CAO82957 CAP45545 CAQ49381 CAR69111 |
| GB | AAA19426 AAB60029 AAC36981 AAM99802 AAY63949 |
| PRF | 2114402X |
| REF | NP_687930 WP_000814511 WP_000814512 WP_006417979 WP_006730246 |
| SP | P61623 P61624 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts