BMRB Entry 6501
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6501
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Title: Backbone 1H, 15N, 13Ca and sidechain 13Cb chemical shift assignments of SARS-CoV Nsp9
Deposition date: 2005-02-12 Original release date: 2005-03-17
Authors: Hu, Yunfei; Lescop, Ewen; Jin, Changwen
Citation: Hu, Yunfei; Lescop, Ewen; Jin, Changwen. "Backbone dynamics of SARS-CoV Nsp9 by NMR spectroscopy" . ., .-..
Assembly members:
Nsp9, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV Taxonomy ID: not available Superkingdom: Viruses Kingdom: Not applicable Genus/species: coronavirus Severe Acute Respiratory Syndrome associated coronavirus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Nsp9: NNELSPVALRQMSCAAGTTQ
TACTDDNALAYYNNSKGGRF
VLALLSDHQDLKWARFPKSD
GTGTIYTELEPPCRFVTDTP
KGPKVKYLYFIKGLNNLNRG
MVLGSLAATVRLQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 189 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nsp9, chain 1 | 1 |
| 2 | Nsp9, chain 2 | 1 |
Entities:
Entity 1, Nsp9, chain 1 113 residues - Formula weight is not available
| 1 | ASN | ASN | GLU | LEU | SER | PRO | VAL | ALA | LEU | ARG | ||||
| 2 | GLN | MET | SER | CYS | ALA | ALA | GLY | THR | THR | GLN | ||||
| 3 | THR | ALA | CYS | THR | ASP | ASP | ASN | ALA | LEU | ALA | ||||
| 4 | TYR | TYR | ASN | ASN | SER | LYS | GLY | GLY | ARG | PHE | ||||
| 5 | VAL | LEU | ALA | LEU | LEU | SER | ASP | HIS | GLN | ASP | ||||
| 6 | LEU | LYS | TRP | ALA | ARG | PHE | PRO | LYS | SER | ASP | ||||
| 7 | GLY | THR | GLY | THR | ILE | TYR | THR | GLU | LEU | GLU | ||||
| 8 | PRO | PRO | CYS | ARG | PHE | VAL | THR | ASP | THR | PRO | ||||
| 9 | LYS | GLY | PRO | LYS | VAL | LYS | TYR | LEU | TYR | PHE | ||||
| 10 | ILE | LYS | GLY | LEU | ASN | ASN | LEU | ASN | ARG | GLY | ||||
| 11 | MET | VAL | LEU | GLY | SER | LEU | ALA | ALA | THR | VAL | ||||
| 12 | ARG | LEU | GLN |
Samples:
sample_1: Nsp9, chain 1, [U-90% 13C; U-90% 15N; U-80% 2H], 0.8 ± 0.2 mM; Nsp9, chain 2, [U-90% 13C; U-90% 15N; U-80% 2H], 0.8 ± 0.2 mM
conditions_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HN(CO)CACB | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAC81346 BAC81347 BAC81360 BAC81361 BAC81374 |
| GB | AAP13439 AAP13442 AAP13566 AAP13575 AAP30028 |
| REF | NP_828849 NP_828850 NP_828867 YP_003858583 |
| SP | P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts