BMRB Entry 6513
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6513
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Title: NMR Structure of the nonstructural Protein 7 (nsP7) from the SARS Corona Virus PubMed: 16188992
Deposition date: 2005-02-18 Original release date: 2007-03-19
Authors: Peti, Wolfgang; Herrmann, Torsten; Johnson, Maggie; Wuthrich, Kurt
Citation: Peti, Wolfgang; Johnson, Maggie; Herrmann, Torsten; Neuman, B.; Buchmeier, M.; Nelson, M.; Joseph, J.; Page, R.; Stevens, R.; Kuhn, P.; Wuthrich, Kurt. "Structural genomics of the SARS coronavirus: NMR structure of the protein nsp7" J. Virol. 79, 12905-12913 (2005).
Assembly members:
non structural protein 7, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: corona virus Taxonomy ID: not available Superkingdom: Viruses Kingdom: not available Genus/species: SARS corona virus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
non structural protein 7: GHSKMSDVKCTSVVLLSVLQ
QLRVESSSKLWAQCVQLHND
ILLAKDTTEAFEKMVSLLSV
LLSMQGAVDINRLCEEMLDN
RATLQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 504 |
| 13C chemical shifts | 323 |
| 15N chemical shifts | 75 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | nsp7, monomer | 1 |
Entities:
Entity 1, nsp7, monomer 85 residues - Formula weight is not available
| 1 | GLY | HIS | SER | LYS | MET | SER | ASP | VAL | LYS | CYS | ||||
| 2 | THR | SER | VAL | VAL | LEU | LEU | SER | VAL | LEU | GLN | ||||
| 3 | GLN | LEU | ARG | VAL | GLU | SER | SER | SER | LYS | LEU | ||||
| 4 | TRP | ALA | GLN | CYS | VAL | GLN | LEU | HIS | ASN | ASP | ||||
| 5 | ILE | LEU | LEU | ALA | LYS | ASP | THR | THR | GLU | ALA | ||||
| 6 | PHE | GLU | LYS | MET | VAL | SER | LEU | LEU | SER | VAL | ||||
| 7 | LEU | LEU | SER | MET | GLN | GLY | ALA | VAL | ASP | ILE | ||||
| 8 | ASN | ARG | LEU | CYS | GLU | GLU | MET | LEU | ASP | ASN | ||||
| 9 | ARG | ALA | THR | LEU | GLN |
Samples:
sample1: non structural protein 7, [U-15N], 2.3 mM; DTT 5 mM
sample2: non structural protein 7, [U-13C; U-15N], 3.2 mM; DTT 5 mM
sample3: non structural protein 7 1 mM; DTT 5 mM
cond1: pH: 7.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1h15nhsqc | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
Software:
CARA v0.9 -
NMR spectrometers:
- BRUKER AVANCE 600 MHz
- BRUKER AVANCE 900 MHz
Related Database Links:
| BMRB | 16981 |
| PDB | |
| DBJ | BAC81346 BAC81347 BAC81360 BAC81361 BAC81374 |
| GB | AAP13439 AAP13442 AAP13566 AAP13575 AAP30028 |
| REF | NP_828849 NP_828850 NP_828865 |
| SP | P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts