BMRB Entry 6688
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6688
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Title: NMR chemical shift entry for protein Rv1980c (MPT64) from M. tuberculosis PubMed: 16222561
Deposition date: 2005-06-13 Original release date: 2015-09-02
Authors: Danahy, Jessica; Potter, Belinda; Geisbrecht, Brian; Laity, John
Citation: Danahy, Jessica; Potter, Belinda; Geisbrecht, Brian; Laity, John. "NMR Assignment of Protein Rv1980c from Mycobacterium Tuberculosis" J. Biomol. NMR 33, 73-73 (2005).
Assembly members:
Rv1980c (MPT64) polypeptide, polymer, 206 residues, 22564 Da.
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 83332 Superkingdom: Eubacteria Kingdom: Not applicable Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Rv1980c (MPT64) polypeptide: MAPKTYCEELKGTDTGQACQ
IQMSDPAYNINISLPSYYPD
QKSLENYIAQTRDKFLSAAT
SSTPREAPYELNITSATYQS
AIPPRGTQAVVLKVYQNAGG
THPTTTYKAFDWDQAYRKPI
TYDTLWQADTDPLPVVFPIV
QGELSKQTGQQVSIAPNAGL
DPVNYQNFAVTNDGVIFFFN
PGELLPEAAGPTQVLVPRSA
IDSMLA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 832 |
| 15N chemical shifts | 199 |
| 1H chemical shifts | 1207 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rv1980c (MPT64) | 1 |
Entities:
Entity 1, Rv1980c (MPT64) 206 residues - 22564 Da.
Sequence of recombinant, mature polypeptide.
| 1 | MET | ALA | PRO | LYS | THR | TYR | CYS | GLU | GLU | LEU | ||||
| 2 | LYS | GLY | THR | ASP | THR | GLY | GLN | ALA | CYS | GLN | ||||
| 3 | ILE | GLN | MET | SER | ASP | PRO | ALA | TYR | ASN | ILE | ||||
| 4 | ASN | ILE | SER | LEU | PRO | SER | TYR | TYR | PRO | ASP | ||||
| 5 | GLN | LYS | SER | LEU | GLU | ASN | TYR | ILE | ALA | GLN | ||||
| 6 | THR | ARG | ASP | LYS | PHE | LEU | SER | ALA | ALA | THR | ||||
| 7 | SER | SER | THR | PRO | ARG | GLU | ALA | PRO | TYR | GLU | ||||
| 8 | LEU | ASN | ILE | THR | SER | ALA | THR | TYR | GLN | SER | ||||
| 9 | ALA | ILE | PRO | PRO | ARG | GLY | THR | GLN | ALA | VAL | ||||
| 10 | VAL | LEU | LYS | VAL | TYR | GLN | ASN | ALA | GLY | GLY | ||||
| 11 | THR | HIS | PRO | THR | THR | THR | TYR | LYS | ALA | PHE | ||||
| 12 | ASP | TRP | ASP | GLN | ALA | TYR | ARG | LYS | PRO | ILE | ||||
| 13 | THR | TYR | ASP | THR | LEU | TRP | GLN | ALA | ASP | THR | ||||
| 14 | ASP | PRO | LEU | PRO | VAL | VAL | PHE | PRO | ILE | VAL | ||||
| 15 | GLN | GLY | GLU | LEU | SER | LYS | GLN | THR | GLY | GLN | ||||
| 16 | GLN | VAL | SER | ILE | ALA | PRO | ASN | ALA | GLY | LEU | ||||
| 17 | ASP | PRO | VAL | ASN | TYR | GLN | ASN | PHE | ALA | VAL | ||||
| 18 | THR | ASN | ASP | GLY | VAL | ILE | PHE | PHE | PHE | ASN | ||||
| 19 | PRO | GLY | GLU | LEU | LEU | PRO | GLU | ALA | ALA | GLY | ||||
| 20 | PRO | THR | GLN | VAL | LEU | VAL | PRO | ARG | SER | ALA | ||||
| 21 | ILE | ASP | SER | MET | LEU | ALA |
Samples:
sample_1: Rv1980c (MPT64) polypeptide, [U-15N; U-13C], 2.0 ± 0.05 mM; MES 20 mM; D2O 5%; NaN3 1 mM; DSS 0.2 mM
conditions_1: pH: 6.85; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N_HSQC | sample_1 | not available | conditions_1 |
| 3D HNCACB | sample_1 | not available | conditions_1 |
| 3D CBCA(CO)NH | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D (HCA)CO(CA)NH | sample_1 | not available | conditions_1 |
| 3D HNHA | sample_1 | not available | conditions_1 |
| 3D 13C-NOESY-HSQC (centered at 43 ppm) | sample_1 | not available | conditions_1 |
| 3D 13C-NOESY-HSQC (centered at 125 ppm) | sample_1 | not available | conditions_1 |
| 3D HCCH-COSY | sample_1 | not available | conditions_1 |
| 3D HCCH-TOCSY | sample_1 | not available | conditions_1 |
| 3D 15N-NOESY-HSQC | sample_1 | not available | conditions_1 |
| 3D HNCA | sample_1 | not available | conditions_1 |
Software:
NMRView v5.0.4 - Data analysis
NMRPipe v97.027.12.56 - Raw spectral data processing
NMR spectrometers:
- Varian Inova 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts