BMRB Entry 7014

Name: NMR assigment of the SARS-CoV protein nsp1
Published: 2006-08-09

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PDB ID: 2gdt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7014
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR assigment of the SARS-CoV protein nsp1 PubMed: 16821128

Deposition date: 2006-03-06 Original release date: 2006-08-09

Authors: Almeida, Marcius; Geralt, Michael; Johnson, Margaret; Saikatendu, Kumar; Joseph, Jeremiah; Subramanian, Vanitha; Stevens, Raymond; Kuhn, Peter; Wuthrich, Kurt

Citation: Almeida, Marcius; Johnson, Margaret; Wuthrich, Kurt. "NMR assignment of the SARS-CoV protein nsp1" J. Biomol. NMR 36, 46-46 (2006).

Assembly members:
leader protein from SARS-CoV, polymer, 116 residues, Formula weight is not available

Natural source: Common Name: SARS-CoV Taxonomy ID: 227859 Superkingdom: Viruses Kingdom: not available Genus/species: Coronavirus SARS coronavirus

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
leader protein from SARS-CoV: GHVQLSLPVLQVRDVLVRGF GDSVEEALSEAREHLKNGTC GLVELEKGVLPQLEQPYVFI KRSDALSTNHGHKVVELVAE MDGIQYGRSGITLGVLVPHV GETPIAYRNVLLRKNG

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	503
15N chemical shifts	116
1H chemical shifts	833

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nsp1	1

Entities:

Entity 1, nsp1 116 residues - Formula weight is not available

1

GLY

HIS

VAL

GLN

LEU

SER

LEU

PRO

VAL

LEU

2

GLN

VAL

ARG

ASP

VAL

LEU

VAL

ARG

GLY

PHE

3

GLY

ASP

SER

VAL

GLU

ALA

LEU

SER

GLU

4

ALA

ARG

GLU

HIS

LEU

LYS

ASN

GLY

THR

CYS

5

GLY

LEU

VAL

GLU

LEU

GLU

LYS

GLY

VAL

LEU

6

PRO

GLN

LEU

GLU

GLN

PRO

TYR

VAL

PHE

ILE

7

LYS

ARG

SER

ASP

ALA

LEU

SER

THR

ASN

HIS

8

GLY

HIS

LYS

VAL

GLU

LEU

VAL

ALA

GLU

9

MET

ASP

GLY

ILE

GLN

TYR

GLY

ARG

SER

GLY

10

ILE

THR

LEU

GLY

VAL

LEU

VAL

PRO

HIS

VAL

11

GLY

GLU

THR

PRO

ILE

ALA

TYR

ARG

ASN

VAL

12

LEU

ARG

LYS

ASN

GLY

Samples:

sample_1: leader protein from SARS-CoV, [U-13C; U-15N], 2 ± 0.2 mM; Na Phosphate 25 mM; NaCl 250 mM; Azide 0.03%; D2O 10%

conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D [15N,1H]-HSQC	sample_1	not available	conditions_1
3D HNCA	sample_1	not available	conditions_1
2D [13C,1H]-HSQC	sample_1	not available	conditions_1
3D HNCO	sample_1	not available	conditions_1
3D HNCACB	sample_1	not available	conditions_1
3D CBCA(CO)NH	sample_1	not available	conditions_1
3D HBHA(CO)NH	sample_1	not available	conditions_1
3D CC(co)NH	sample_1	not available	conditions_1
3D 13C-[1H,1H] NOESY	sample_1	not available	conditions_1
3D 15N-[1H,1H] NOESY	sample_1	not available	conditions_1

Software:

CARA v1.4, . - processing

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2GDT 2HSX
DBJ	BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB	AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF	NP_828849 NP_828850 NP_828860
SP	P0C6F8 P0C6U8 P0C6W2 P0C6X7

Biological Magnetic Resonance Data Bank