BMRB Entry 7059
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7059
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Title: 1H, 13C, and 15N Resonance Assignments of the Pyrazinamidase from Mycobacterium Tuberculosis PubMed: 17131033
Deposition date: 2006-04-10 Original release date: 2006-10-02
Authors: Son, Woo-Sung; Kim, Won-Je; Kim, Yong-Jin; Suh, Se-Won; Lee, Bong-Jin
Citation: Son, Woo-Sung; Kim, Won-Je; Kim, Yong-Jin; Suh, Se-Won; Ikegami, Takahisa; Akutsu, Hideo; Lee, Bong-Jin. "1H, 13C and 15N Resonance Assignments of the Pyrazinamidase from Mycobacterium Tuberculosis" J. Biomol. NMR 36, 60-60 (2006).
Assembly members:
Pyrazinamidase, polymer, 186 residues, 19604.6 Da.
Natural source: Common Name: Mycobacterium Tuberculosis Taxonomy ID: 1773 Superkingdom: Eubacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Pyrazinamidase: MRALIIVDVQNDFCEGGSLA
VTGGAALARAISDYLAEAAD
YHHVVATKDFHIDPGDHFSG
TPDYSSSWPPHCVSGTPGAD
FHPSLDTSAIEAVFYKGAYT
GAYSGFEGVDENGTPLLNWL
RQRGVDEVDVVGIATDHCVR
QTAEDAVRNGLATRVLVDLT
AGVSADTTVAALEEMRTASV
ELVCSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 679 |
| 15N chemical shifts | 178 |
| 1H chemical shifts | 1110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pyrazinamidase | 1 |
Entities:
Entity 1, Pyrazinamidase 186 residues - 19604.6 Da.
| 1 | MET | ARG | ALA | LEU | ILE | ILE | VAL | ASP | VAL | GLN | ||||
| 2 | ASN | ASP | PHE | CYS | GLU | GLY | GLY | SER | LEU | ALA | ||||
| 3 | VAL | THR | GLY | GLY | ALA | ALA | LEU | ALA | ARG | ALA | ||||
| 4 | ILE | SER | ASP | TYR | LEU | ALA | GLU | ALA | ALA | ASP | ||||
| 5 | TYR | HIS | HIS | VAL | VAL | ALA | THR | LYS | ASP | PHE | ||||
| 6 | HIS | ILE | ASP | PRO | GLY | ASP | HIS | PHE | SER | GLY | ||||
| 7 | THR | PRO | ASP | TYR | SER | SER | SER | TRP | PRO | PRO | ||||
| 8 | HIS | CYS | VAL | SER | GLY | THR | PRO | GLY | ALA | ASP | ||||
| 9 | PHE | HIS | PRO | SER | LEU | ASP | THR | SER | ALA | ILE | ||||
| 10 | GLU | ALA | VAL | PHE | TYR | LYS | GLY | ALA | TYR | THR | ||||
| 11 | GLY | ALA | TYR | SER | GLY | PHE | GLU | GLY | VAL | ASP | ||||
| 12 | GLU | ASN | GLY | THR | PRO | LEU | LEU | ASN | TRP | LEU | ||||
| 13 | ARG | GLN | ARG | GLY | VAL | ASP | GLU | VAL | ASP | VAL | ||||
| 14 | VAL | GLY | ILE | ALA | THR | ASP | HIS | CYS | VAL | ARG | ||||
| 15 | GLN | THR | ALA | GLU | ASP | ALA | VAL | ARG | ASN | GLY | ||||
| 16 | LEU | ALA | THR | ARG | VAL | LEU | VAL | ASP | LEU | THR | ||||
| 17 | ALA | GLY | VAL | SER | ALA | ASP | THR | THR | VAL | ALA | ||||
| 18 | ALA | LEU | GLU | GLU | MET | ARG | THR | ALA | SER | VAL | ||||
| 19 | GLU | LEU | VAL | CYS | SER | SER |
Samples:
sample_1: Pyrazinamidase, [U-13C; U-15N; 70%-2H], 0.8 ± 0.1 mM; DTT 10 mM; NaN3 1 mM
sample_2: Pyrazinamidase, [U-13C; U-15N], 0.8 ± 0.1 mM; DTT 10 mM; NaN3 1 mM
sample_3: Pyrazinamidase, [U-15N], 0.8 ± 0.1 mM; DTT 10 mM; NaN3 1 mM
conditions_1: pH: 6.7; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | not available | not available | conditions_1 |
| 1H15N_TROSY | not available | not available | conditions_1 |
| 3D TROSY-HNCA | not available | not available | conditions_1 |
| 3D TROSY-HN(CO)CA | not available | not available | conditions_1 |
| 3D TROSY-HNCACB | not available | not available | conditions_1 |
| 3D TROSY-HN(CO)CACB | not available | not available | conditions_1 |
| 3D TROSY-HN(CA)CO | not available | not available | conditions_1 |
| 3D TROSY-HNCO | not available | not available | conditions_1 |
| 3D CC(CO)NH | not available | not available | conditions_1 |
| 3D (H)CC(CO)NH | not available | not available | conditions_1 |
| 3D HNHA | not available | not available | conditions_1 |
| 3D HBHA(CO)NH | not available | not available | conditions_1 |
| 3D 15N-edited NOESY-TROSY | not available | not available | conditions_1 |
| 3D 13C-edited NOESY-HSQC | not available | not available | conditions_1 |
| 3D 13C-edited TOCSY-HSQC | not available | not available | conditions_1 |
| 3D HCCH-COSY | not available | not available | conditions_1 |
| 3D HCCH-TOCSY | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAH26342 BAL66049 BAO48146 BAQ06101 GAA45748 |
| EMBL | CAD96922 CAL72050 CCC27128 CCC44398 CCC64640 |
| GB | AAB37768 AAK38743 AAK46382 AAV33179 AAV33180 |
| REF | NP_216559 NP_336568 NP_855719 WP_003410243 WP_003906725 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts