data_6698 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 6698 _Entry.Title ; The structured core of a largely unstructured protein, malarial merozoite surface protein 2 (MSP2), is amyloidogenic ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2005-06-21 _Entry.Accession_date 2005-06-22 _Entry.Last_release_date 2008-10-27 _Entry.Original_release_date 2008-10-27 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version . _Entry.Experimental_method NMR _Entry.Experimental_method_subtype . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 David Keizer . W . 6698 2 Christopher Adda . G . 6698 3 Vince Murphy . J . 6698 4 Michael Rizkalla . M . 6698 5 David Jackson . C . 6698 6 Robin Anders . F . 6698 7 Raymond Norton . S . 6698 stop_ loop_ _Entry_src.ID _Entry_src.Project_name _Entry_src.Organization_full_name _Entry_src.Organization_initials _Entry_src.Entry_ID . . WEHI . 6698 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 6698 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '1H chemical shifts' 145 6698 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2008-10-27 2005-06-21 original author . 6698 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 6698 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 17883245 _Citation.Full_citation . _Citation.Title 'A partially structured region of a largely unstructured protein, Plasmodium falciparum merozoite surface protein 2 (MSP2), forms amyloid-like fibrils' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Pept. Sci.' _Citation.Journal_name_full . _Citation.Journal_volume 13 _Citation.Journal_issue 12 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 839 _Citation.Page_last 848 _Citation.Year 2007 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 X. Yang . . . 6698 1 2 Christopher Adda . G. . 6698 1 3 David Keizer . W. . 6698 1 4 Vince Murphy . J. . 6698 1 5 M. Rizkalla . M. . 6698 1 6 M. Perugini . A. . 6698 1 7 David Jackson . C. . 6698 1 8 Robin Anders . F. . 6698 1 9 Raymond Norton . S. . 6698 1 stop_ loop_ _Citation_keyword.Keyword _Citation_keyword.Entry_ID _Citation_keyword.Citation_ID malaria 6698 1 MSP2 6698 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 6698 _Assembly.ID 1 _Assembly.Name 'MSP2 peptide' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 1 _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites no _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state 'not present' _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'MSP2 peptide' 1 $MSP2_N-terminal_region . . yes native no no . . . 6698 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_MSP2_N-terminal_region _Entity.Sf_category entity _Entity.Sf_framecode MSP2_N-terminal_region _Entity.Entry_ID 6698 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name 'MSP2 N-terminal region' _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; IKNESKYSNTFINNAYNMSI RRSMA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 25 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-28 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 15075 . 1-25MSP2 . . . . . 100.00 28 100.00 100.00 4.98e-07 . . . . 6698 1 2 no BMRB 15590 . MSP2 . . . . . 100.00 222 100.00 100.00 6.44e-08 . . . . 6698 1 3 no BMRB 25202 . entity . . . . . 80.00 20 100.00 100.00 1.78e-03 . . . . 6698 1 4 no PDB 2MU8 . "Distorting Malaria Peptide Backbone Structure To Enable Fitting Into Mhc Class Ii Molecules Renders Modified Peptides Immunogen" . . . . . 80.00 20 100.00 100.00 1.78e-03 . . . . 6698 1 5 no EMBL CAA37829 . "merozoite surface antigen 2 [Plasmodium falciparum]" . . . . . 96.00 287 100.00 100.00 5.50e-07 . . . . 6698 1 6 no EMBL CAA37830 . "merozoite surface antigen 2 [Plasmodium falciparum]" . . . . . 96.00 302 100.00 100.00 7.19e-07 . . . . 6698 1 7 no EMBL CAA53701 . "glycosylated and myristilated smaller surface antigen [Plasmodium falciparum]" . . . . . 100.00 278 100.00 100.00 1.95e-07 . . . . 6698 1 8 no EMBL CAA53946 . "glycosylated and myristilated smaller surface antigen [Plasmodium falciparum]" . . . . . 100.00 256 100.00 100.00 3.17e-07 . . . . 6698 1 9 no EMBL CAA60699 . "Merozoite surface antigen 2 [Plasmodium falciparum]" . . . . . 100.00 264 100.00 100.00 4.25e-07 . . . . 6698 1 10 no GB AAA19735 . "merozoite surface antigen-2, partial [Plasmodium falciparum]" . . . . . 100.00 264 100.00 100.00 1.92e-07 . . . . 6698 1 11 no GB AAA19737 . "merozoite surface antigen 2, partial [Plasmodium falciparum]" . . . . . 96.00 293 100.00 100.00 3.95e-07 . . . . 6698 1 12 no GB AAA19738 . "merozoite surface antigen 2, partial [Plasmodium falciparum]" . . . . . 100.00 248 100.00 100.00 1.48e-07 . . . . 6698 1 13 no GB AAA19739 . "merozoite surface antigen 2 [Plasmodium falciparum]" . . . . . 96.00 223 100.00 100.00 5.46e-04 . . . . 6698 1 14 no GB AAA19740 . "merozoite surface antigen 2, partial [Plasmodium falciparum]" . . . . . 96.00 291 100.00 100.00 1.70e-07 . . . . 6698 1 15 no PIR B45632 . "merozoite surface antigen 2 - malaria parasite (Plasmodium falciparum)" . . . . . 96.00 286 100.00 100.00 6.96e-07 . . . . 6698 1 16 no PIR C39112 . "merozoite 45K surface antigen precursor - malaria parasite (Plasmodium falciparum) (isolate K1)" . . . . . 100.00 280 100.00 100.00 3.66e-07 . . . . 6698 1 17 no PRF 2023165A . "surface antigen" . . . . . 100.00 278 100.00 100.00 1.95e-07 . . . . 6698 1 18 no PRF 2023165B . "surface antigen" . . . . . 100.00 256 100.00 100.00 3.17e-07 . . . . 6698 1 19 no REF XP_001349578 . "merozoite surface protein 2 precursor [Plasmodium falciparum 3D7]" . . . . . 100.00 272 100.00 100.00 2.04e-07 . . . . 6698 1 20 no SP P19260 . "RecName: Full=Merozoite surface antigen 2, allelic form 2; Short=MSA-2; AltName: Full=Membrane protein PF7; Flags: Precursor [P" . . . . . 96.00 287 100.00 100.00 5.50e-07 . . . . 6698 1 21 no SP P19599 . "RecName: Full=Merozoite surface antigen 2; Short=MSA-2; AltName: Full=AG513; AltName: Full=Merozoite 45 kDa surface antigen; Fl" . . . . . 100.00 264 100.00 100.00 4.16e-07 . . . . 6698 1 22 no SP P50496 . "RecName: Full=Merozoite surface antigen 2; Short=MSA-2; Flags: Precursor [Plasmodium falciparum 311]" . . . . . 96.00 286 100.00 100.00 6.89e-07 . . . . 6698 1 23 no SP P50497 . "RecName: Full=Merozoite surface antigen 2; Short=MSA-2; Flags: Precursor [Plasmodium falciparum KF1916]" . . . . . 100.00 274 100.00 100.00 2.33e-07 . . . . 6698 1 24 no SP P50498 . "RecName: Full=Merozoite surface antigen 2; Short=MSA-2; AltName: Full=45 kDa merozoite surface antigen; Flags: Precursor [Plasm" . . . . . 100.00 272 100.00 100.00 2.04e-07 . . . . 6698 1 stop_ loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID Unknown 6698 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . ILE . 6698 1 2 . LYS . 6698 1 3 . ASN . 6698 1 4 . GLU . 6698 1 5 . SER . 6698 1 6 . LYS . 6698 1 7 . TYR . 6698 1 8 . SER . 6698 1 9 . ASN . 6698 1 10 . THR . 6698 1 11 . PHE . 6698 1 12 . ILE . 6698 1 13 . ASN . 6698 1 14 . ASN . 6698 1 15 . ALA . 6698 1 16 . TYR . 6698 1 17 . ASN . 6698 1 18 . MET . 6698 1 19 . SER . 6698 1 20 . ILE . 6698 1 21 . ARG . 6698 1 22 . ARG . 6698 1 23 . SER . 6698 1 24 . MET . 6698 1 25 . ALA . 6698 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ILE 1 1 6698 1 . LYS 2 2 6698 1 . ASN 3 3 6698 1 . GLU 4 4 6698 1 . SER 5 5 6698 1 . LYS 6 6 6698 1 . TYR 7 7 6698 1 . SER 8 8 6698 1 . ASN 9 9 6698 1 . THR 10 10 6698 1 . PHE 11 11 6698 1 . ILE 12 12 6698 1 . ASN 13 13 6698 1 . ASN 14 14 6698 1 . ALA 15 15 6698 1 . TYR 16 16 6698 1 . ASN 17 17 6698 1 . MET 18 18 6698 1 . SER 19 19 6698 1 . ILE 20 20 6698 1 . ARG 21 21 6698 1 . ARG 22 22 6698 1 . SER 23 23 6698 1 . MET 24 24 6698 1 . ALA 25 25 6698 1 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 6698 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $MSP2_N-terminal_region . 5833 . no . 'Plasmodium falciparum' . . Eukaryota . Plasmodium falciparum . . . . . . . . . . . . . . . . . . . . . 6698 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 6698 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $MSP2_N-terminal_region . 'chemical synthesis' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6698 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 6698 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system . _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'MSP2 N-terminal region' . 1 $assembly 1 $MSP2_N-terminal_region . protein . . . nM . . . . 6698 1 stop_ save_ ####################### # Sample conditions # ####################### save_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode conditions_1 _Sample_condition_list.Entry_ID 6698 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 3.4 0.1 pH 6698 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600MHz_spectrometer _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode 600MHz_spectrometer _NMR_spectrometer.Entry_ID 6698 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_500MHz_spectrometer _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode 500MHz_spectrometer _NMR_spectrometer.Entry_ID 6698 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ ############################# # NMR applied experiments # ############################# save_NMR_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode NMR_experiment_list _Experiment_list.Entry_ID 6698 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 . no . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6698 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_referencing _Chem_shift_reference.Entry_ID 6698 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Indirect_shift_ratio_cit_ID _Chem_shift_ref.Indirect_shift_ratio_cit_label _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Correction_val_cit_ID _Chem_shift_ref.Correction_val_cit_label _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0.0 internal direct 1.0 . . . . . . . . . 6698 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode chem_shift_list_1 _Assigned_chem_shift_list.Entry_ID 6698 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_referencing _Assigned_chem_shift_list.Chem_shift_1H_err 0.02 _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 . 1 $sample_1 isotropic 6698 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 2 2 LYS H H 1 8.61 0.02 . 1 . . . . 2 LYS H . 6698 1 2 . 1 1 2 2 LYS HA H 1 4.32 0.02 . 1 . . . . 2 LYS HA . 6698 1 3 . 1 1 2 2 LYS HB2 H 1 1.75 0.02 . 1 . . . . 2 LYS HB2 . 6698 1 4 . 1 1 2 2 LYS HB3 H 1 1.75 0.02 . 1 . . . . 2 LYS HB3 . 6698 1 5 . 1 1 2 2 LYS HG2 H 1 1.37 0.02 . 1 . . . . 2 LYS HG2 . 6698 1 6 . 1 1 2 2 LYS HG3 H 1 1.37 0.02 . 1 . . . . 2 LYS HG3 . 6698 1 7 . 1 1 3 3 ASN H H 1 8.62 0.02 . 1 . . . . 3 ASN H . 6698 1 8 . 1 1 3 3 ASN HA H 1 5.65 0.02 . 1 . . . . 3 ASN HA . 6698 1 9 . 1 1 3 3 ASN HB2 H 1 2.71 0.02 . 2 . . . . 3 ASN HB2 . 6698 1 10 . 1 1 3 3 ASN HB3 H 1 2.80 0.02 . 2 . . . . 3 ASN HB3 . 6698 1 11 . 1 1 3 3 ASN HD21 H 1 7.52 0.02 . 1 . . . . 3 ASN HD21 . 6698 1 12 . 1 1 3 3 ASN HD22 H 1 7.05 0.02 . 1 . . . . 3 ASN HD22 . 6698 1 13 . 1 1 4 4 GLU H H 1 8.47 0.02 . 1 . . . . 4 GLU H . 6698 1 14 . 1 1 4 4 GLU HA H 1 4.31 0.02 . 1 . . . . 4 GLU HA . 6698 1 15 . 1 1 4 4 GLU HB2 H 1 1.89 0.02 . 1 . . . . 4 GLU HB2 . 6698 1 16 . 1 1 4 4 GLU HB3 H 1 1.89 0.02 . 1 . . . . 4 GLU HB3 . 6698 1 17 . 1 1 4 4 GLU HG2 H 1 2.25 0.02 . 1 . . . . 4 GLU HG2 . 6698 1 18 . 1 1 4 4 GLU HG3 H 1 2.25 0.02 . 1 . . . . 4 GLU HG3 . 6698 1 19 . 1 1 5 5 SER H H 1 8.41 0.02 . 1 . . . . 5 SER H . 6698 1 20 . 1 1 5 5 SER HA H 1 4.39 0.02 . 1 . . . . 5 SER HA . 6698 1 21 . 1 1 5 5 SER HB2 H 1 3.85 0.02 . 1 . . . . 5 SER HB2 . 6698 1 22 . 1 1 5 5 SER HB3 H 1 3.85 0.02 . 1 . . . . 5 SER HB3 . 6698 1 23 . 1 1 6 6 LYS H H 1 8.10 0.02 . 1 . . . . 6 LYS H . 6698 1 24 . 1 1 6 6 LYS HA H 1 4.17 0.02 . 1 . . . . 6 LYS HA . 6698 1 25 . 1 1 6 6 LYS HB2 H 1 1.36 0.02 . 1 . . . . 6 LYS HB2 . 6698 1 26 . 1 1 6 6 LYS HB3 H 1 1.36 0.02 . 1 . . . . 6 LYS HB3 . 6698 1 27 . 1 1 6 6 LYS HG2 H 1 1.17 0.02 . 1 . . . . 6 LYS HG2 . 6698 1 28 . 1 1 6 6 LYS HG3 H 1 1.17 0.02 . 1 . . . . 6 LYS HG3 . 6698 1 29 . 1 1 7 7 TYR H H 1 8.04 0.02 . 1 . . . . 7 TYR H . 6698 1 30 . 1 1 7 7 TYR HA H 1 4.59 0.02 . 1 . . . . 7 TYR HA . 6698 1 31 . 1 1 7 7 TYR HB2 H 1 2.85 0.02 . 1 . . . . 7 TYR HB2 . 6698 1 32 . 1 1 7 7 TYR HB3 H 1 3.07 0.02 . 1 . . . . 7 TYR HB3 . 6698 1 33 . 1 1 7 7 TYR HD1 H 1 7.08 0.02 . 1 . . . . 7 TYR HD1 . 6698 1 34 . 1 1 8 8 SER H H 1 8.08 0.02 . 1 . . . . 8 SER H . 6698 1 35 . 1 1 8 8 SER HA H 1 4.37 0.02 . 1 . . . . 8 SER HA . 6698 1 36 . 1 1 8 8 SER HB2 H 1 3.85 0.02 . 1 . . . . 8 SER HB2 . 6698 1 37 . 1 1 8 8 SER HB3 H 1 3.85 0.02 . 1 . . . . 8 SER HB3 . 6698 1 38 . 1 1 9 9 ASN H H 1 8.40 0.02 . 1 . . . . 9 ASN H . 6698 1 39 . 1 1 9 9 ASN HA H 1 4.70 0.02 . 1 . . . . 9 ASN HA . 6698 1 40 . 1 1 9 9 ASN HB2 H 1 2.76 0.02 . 1 . . . . 9 ASN HB2 . 6698 1 41 . 1 1 9 9 ASN HB3 H 1 2.76 0.02 . 1 . . . . 9 ASN HB3 . 6698 1 42 . 1 1 10 10 THR H H 1 7.98 0.02 . 1 . . . . 10 THR H . 6698 1 43 . 1 1 10 10 THR HA H 1 4.19 0.02 . 1 . . . . 10 THR HA . 6698 1 44 . 1 1 10 10 THR HB H 1 4.09 0.02 . 1 . . . . 10 THR HB . 6698 1 45 . 1 1 10 10 THR HG21 H 1 1.08 0.02 . 1 . . . . 10 THR HG2 . 6698 1 46 . 1 1 10 10 THR HG22 H 1 1.08 0.02 . 1 . . . . 10 THR HG2 . 6698 1 47 . 1 1 10 10 THR HG23 H 1 1.08 0.02 . 1 . . . . 10 THR HG2 . 6698 1 48 . 1 1 11 11 PHE H H 1 8.11 0.02 . 1 . . . . 11 PHE H . 6698 1 49 . 1 1 11 11 PHE HA H 1 4.56 0.02 . 1 . . . . 11 PHE HA . 6698 1 50 . 1 1 11 11 PHE HB2 H 1 3.01 0.02 . 1 . . . . 11 PHE HB2 . 6698 1 51 . 1 1 11 11 PHE HB3 H 1 3.09 0.02 . 1 . . . . 11 PHE HB3 . 6698 1 52 . 1 1 11 11 PHE HD1 H 1 7.21 0.02 . 1 . . . . 11 PHE HD1 . 6698 1 53 . 1 1 11 11 PHE HE1 H 1 7.32 0.02 . 1 . . . . 11 PHE HE1 . 6698 1 54 . 1 1 11 11 PHE HZ H 1 7.29 0.02 . 1 . . . . 11 PHE HZ . 6698 1 55 . 1 1 12 12 ILE H H 1 7.91 0.02 . 1 . . . . 12 ILE H . 6698 1 56 . 1 1 12 12 ILE HA H 1 4.01 0.02 . 1 . . . . 12 ILE HA . 6698 1 57 . 1 1 12 12 ILE HB H 1 1.74 0.02 . 1 . . . . 12 ILE HB . 6698 1 58 . 1 1 12 12 ILE HG12 H 1 1.11 0.02 . 1 . . . . 12 ILE HG12 . 6698 1 59 . 1 1 12 12 ILE HG13 H 1 1.40 0.02 . 1 . . . . 12 ILE HG13 . 6698 1 60 . 1 1 12 12 ILE HG21 H 1 0.82 0.02 . 1 . . . . 12 ILE HG2 . 6698 1 61 . 1 1 12 12 ILE HG22 H 1 0.82 0.02 . 1 . . . . 12 ILE HG2 . 6698 1 62 . 1 1 12 12 ILE HG23 H 1 0.82 0.02 . 1 . . . . 12 ILE HG2 . 6698 1 63 . 1 1 12 12 ILE HD11 H 1 0.78 0.02 . 1 . . . . 12 ILE HD1 . 6698 1 64 . 1 1 12 12 ILE HD12 H 1 0.78 0.02 . 1 . . . . 12 ILE HD1 . 6698 1 65 . 1 1 12 12 ILE HD13 H 1 0.78 0.02 . 1 . . . . 12 ILE HD1 . 6698 1 66 . 1 1 13 13 ASN H H 1 8.30 0.02 . 1 . . . . 13 ASN H . 6698 1 67 . 1 1 13 13 ASN HA H 1 4.61 0.02 . 1 . . . . 13 ASN HA . 6698 1 68 . 1 1 13 13 ASN HB2 H 1 2.69 0.02 . 1 . . . . 13 ASN HB2 . 6698 1 69 . 1 1 13 13 ASN HB3 H 1 2.81 0.02 . 1 . . . . 13 ASN HB3 . 6698 1 70 . 1 1 13 13 ASN HD21 H 1 6.92 0.02 . 1 . . . . 13 ASN HD21 . 6698 1 71 . 1 1 13 13 ASN HD22 H 1 7.59 0.02 . 1 . . . . 13 ASN HD22 . 6698 1 72 . 1 1 14 14 ASN H H 1 8.28 0.02 . 1 . . . . 14 ASN H . 6698 1 73 . 1 1 14 14 ASN HA H 1 4.64 0.02 . 1 . . . . 14 ASN HA . 6698 1 74 . 1 1 14 14 ASN HB2 H 1 2.82 0.02 . 1 . . . . 14 ASN HB2 . 6698 1 75 . 1 1 14 14 ASN HB3 H 1 2.82 0.02 . 1 . . . . 14 ASN HB3 . 6698 1 76 . 1 1 14 14 ASN HD21 H 1 7.05 0.02 . 1 . . . . 14 ASN HD21 . 6698 1 77 . 1 1 14 14 ASN HD22 H 1 7.52 0.02 . 1 . . . . 14 ASN HD22 . 6698 1 78 . 1 1 15 15 ALA H H 1 8.14 0.02 . 1 . . . . 15 ALA H . 6698 1 79 . 1 1 15 15 ALA HA H 1 4.14 0.02 . 1 . . . . 15 ALA HA . 6698 1 80 . 1 1 15 15 ALA HB1 H 1 1.26 0.02 . 1 . . . . 15 ALA HB . 6698 1 81 . 1 1 15 15 ALA HB2 H 1 1.26 0.02 . 1 . . . . 15 ALA HB . 6698 1 82 . 1 1 15 15 ALA HB3 H 1 1.26 0.02 . 1 . . . . 15 ALA HB . 6698 1 83 . 1 1 16 16 TYR H H 1 8.01 0.02 . 1 . . . . 16 TYR H . 6698 1 84 . 1 1 16 16 TYR HA H 1 4.40 0.02 . 1 . . . . 16 TYR HA . 6698 1 85 . 1 1 16 16 TYR HB2 H 1 2.95 0.02 . 1 . . . . 16 TYR HB2 . 6698 1 86 . 1 1 16 16 TYR HB3 H 1 3.00 0.02 . 1 . . . . 16 TYR HB3 . 6698 1 87 . 1 1 16 16 TYR HD1 H 1 7.11 0.02 . 1 . . . . 16 TYR HD1 . 6698 1 88 . 1 1 17 17 ASN H H 1 8.14 0.02 . 1 . . . . 17 ASN H . 6698 1 89 . 1 1 17 17 ASN HA H 1 4.53 0.02 . 1 . . . . 17 ASN HA . 6698 1 90 . 1 1 17 17 ASN HB2 H 1 2.79 0.02 . 1 . . . . 17 ASN HB2 . 6698 1 91 . 1 1 17 17 ASN HB3 H 1 2.79 0.02 . 1 . . . . 17 ASN HB3 . 6698 1 92 . 1 1 17 17 ASN HD21 H 1 6.94 0.02 . 1 . . . . 17 ASN HD21 . 6698 1 93 . 1 1 17 17 ASN HD22 H 1 7.62 0.02 . 1 . . . . 17 ASN HD22 . 6698 1 94 . 1 1 18 18 MET H H 1 8.16 0.02 . 1 . . . . 18 MET H . 6698 1 95 . 1 1 18 18 MET HA H 1 4.47 0.02 . 1 . . . . 18 MET HA . 6698 1 96 . 1 1 18 18 MET HB2 H 1 2.08 0.02 . 1 . . . . 18 MET HB2 . 6698 1 97 . 1 1 18 18 MET HB3 H 1 2.08 0.02 . 1 . . . . 18 MET HB3 . 6698 1 98 . 1 1 18 18 MET HG2 H 1 2.56 0.02 . 1 . . . . 18 MET HG2 . 6698 1 99 . 1 1 18 18 MET HG3 H 1 2.65 0.02 . 1 . . . . 18 MET HG3 . 6698 1 100 . 1 1 18 18 MET HE1 H 1 2.08 0.02 . 1 . . . . 18 MET HE . 6698 1 101 . 1 1 18 18 MET HE2 H 1 2.08 0.02 . 1 . . . . 18 MET HE . 6698 1 102 . 1 1 18 18 MET HE3 H 1 2.08 0.02 . 1 . . . . 18 MET HE . 6698 1 103 . 1 1 19 19 SER H H 1 8.00 0.02 . 1 . . . . 19 SER H . 6698 1 104 . 1 1 19 19 SER HA H 1 4.35 0.02 . 1 . . . . 19 SER HA . 6698 1 105 . 1 1 19 19 SER HB2 H 1 3.85 0.02 . 1 . . . . 19 SER HB2 . 6698 1 106 . 1 1 19 19 SER HB3 H 1 3.85 0.02 . 1 . . . . 19 SER HB3 . 6698 1 107 . 1 1 20 20 ILE H H 1 7.78 0.02 . 1 . . . . 20 ILE H . 6698 1 108 . 1 1 20 20 ILE HA H 1 4.09 0.02 . 1 . . . . 20 ILE HA . 6698 1 109 . 1 1 20 20 ILE HB H 1 1.83 0.02 . 1 . . . . 20 ILE HB . 6698 1 110 . 1 1 20 20 ILE HG12 H 1 1.12 0.02 . 1 . . . . 20 ILE HG12 . 6698 1 111 . 1 1 20 20 ILE HG13 H 1 1.37 0.02 . 1 . . . . 20 ILE HG13 . 6698 1 112 . 1 1 20 20 ILE HG21 H 1 0.82 0.02 . 1 . . . . 20 ILE HG2 . 6698 1 113 . 1 1 20 20 ILE HG22 H 1 0.82 0.02 . 1 . . . . 20 ILE HG2 . 6698 1 114 . 1 1 20 20 ILE HG23 H 1 0.82 0.02 . 1 . . . . 20 ILE HG2 . 6698 1 115 . 1 1 21 21 ARG H H 1 8.30 0.02 . 1 . . . . 21 ARG H . 6698 1 116 . 1 1 21 21 ARG HA H 1 4.33 0.02 . 1 . . . . 21 ARG HA . 6698 1 117 . 1 1 21 21 ARG HB2 H 1 1.80 0.02 . 1 . . . . 21 ARG HB2 . 6698 1 118 . 1 1 21 21 ARG HB3 H 1 1.87 0.02 . 1 . . . . 21 ARG HB3 . 6698 1 119 . 1 1 21 21 ARG HG2 H 1 1.64 0.02 . 1 . . . . 21 ARG HG2 . 6698 1 120 . 1 1 21 21 ARG HG3 H 1 1.64 0.02 . 1 . . . . 21 ARG HG3 . 6698 1 121 . 1 1 21 21 ARG HD2 H 1 3.19 0.02 . 1 . . . . 21 ARG HD2 . 6698 1 122 . 1 1 21 21 ARG HD3 H 1 3.19 0.02 . 1 . . . . 21 ARG HD3 . 6698 1 123 . 1 1 22 22 ARG H H 1 8.30 0.02 . 1 . . . . 22 ARG H . 6698 1 124 . 1 1 22 22 ARG HA H 1 4.33 0.02 . 1 . . . . 22 ARG HA . 6698 1 125 . 1 1 22 22 ARG HB2 H 1 1.80 0.02 . 1 . . . . 22 ARG HB2 . 6698 1 126 . 1 1 22 22 ARG HB3 H 1 1.87 0.02 . 1 . . . . 22 ARG HB3 . 6698 1 127 . 1 1 22 22 ARG HG2 H 1 1.64 0.02 . 1 . . . . 22 ARG HG2 . 6698 1 128 . 1 1 22 22 ARG HG3 H 1 1.64 0.02 . 1 . . . . 22 ARG HG3 . 6698 1 129 . 1 1 22 22 ARG HD2 H 1 3.19 0.02 . 1 . . . . 22 ARG HD2 . 6698 1 130 . 1 1 22 22 ARG HD3 H 1 3.19 0.02 . 1 . . . . 22 ARG HD3 . 6698 1 131 . 1 1 23 23 SER H H 1 8.20 0.02 . 1 . . . . 23 SER H . 6698 1 132 . 1 1 23 23 SER HA H 1 4.42 0.02 . 1 . . . . 23 SER HA . 6698 1 133 . 1 1 23 23 SER HB2 H 1 3.90 0.02 . 1 . . . . 23 SER HB2 . 6698 1 134 . 1 1 23 23 SER HB3 H 1 3.90 0.02 . 1 . . . . 23 SER HB3 . 6698 1 135 . 1 1 24 24 MET H H 1 8.39 0.02 . 1 . . . . 24 MET H . 6698 1 136 . 1 1 24 24 MET HA H 1 4.51 0.02 . 1 . . . . 24 MET HA . 6698 1 137 . 1 1 24 24 MET HB2 H 1 2.02 0.02 . 1 . . . . 24 MET HB2 . 6698 1 138 . 1 1 24 24 MET HB3 H 1 2.13 0.02 . 1 . . . . 24 MET HB3 . 6698 1 139 . 1 1 24 24 MET HG2 H 1 2.56 0.02 . 1 . . . . 24 MET HG2 . 6698 1 140 . 1 1 24 24 MET HG3 H 1 2.63 0.02 . 1 . . . . 24 MET HG3 . 6698 1 141 . 1 1 25 25 ALA H H 1 8.20 0.02 . 1 . . . . 25 ALA H . 6698 1 142 . 1 1 25 25 ALA HA H 1 4.37 0.02 . 1 . . . . 25 ALA HA . 6698 1 143 . 1 1 25 25 ALA HB1 H 1 1.34 0.02 . 1 . . . . 25 ALA HB . 6698 1 144 . 1 1 25 25 ALA HB2 H 1 1.34 0.02 . 1 . . . . 25 ALA HB . 6698 1 145 . 1 1 25 25 ALA HB3 H 1 1.34 0.02 . 1 . . . . 25 ALA HB . 6698 1 stop_ save_