data_7289 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein ; _BMRB_accession_number 7289 _BMRB_flat_file_name bmr7289.str _Entry_type original _Submission_date 2006-09-06 _Accession_date 2006-09-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Assigned 1H chemical shifts of the N-terminal fragment PB(1-40) of the PB1-F2 protein' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bruns Karsten . . 2 Studtrucker Nicole . . 3 Sharma Alok . . 4 Fossen Torgils . . 5 Mitzner David . . 6 Eissmann Andre . . 7 Tessmer Uwe . . 8 Henklein Peter . . 9 Wray Victor . . 10 Schubert Ulrich . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 251 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-01-08 update author 'update the entry citation' 2006-10-31 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 7258 'NMR data of the C-terminal fragment PB(50-87) of the PB1-F2 protein' 7290 'NMR data of the central fragment PB(30-70) of the PB1-F2 protein' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17052982 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bruns Karsten . . 2 Studtrucker Nicole . . 3 Sharma Alok . . 4 Fossen Torgils . . 5 Mitzner David . . 6 Eissmann Andre . . 7 Tessmer Uwe . . 8 Roder R. . . 9 Henklein Peter . . 10 Wray Victor . . 11 Schubert Ulrich . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 282 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 353 _Page_last 363 _Year 2006 _Details . loop_ _Keyword 'Influenza A virus' PB1-F2 'Structure and oligomerization' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name PB(1-40) _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label PB(1-40) $PB(1-40) stop_ _System_molecular_weight 4592 _System_physical_state native _System_oligomer_state protein _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details 'N-terminal fragment of influenza A virus PB1-F2 protein' save_ ######################## # Monomeric polymers # ######################## save_PB(1-40) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common PB(1-40) _Molecular_mass 4592 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 40 _Mol_residue_sequence ; MGQEQDTPWILSTGHISTQK RQDGQQTPKLEHRNSTRLMG ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLY 3 GLN 4 GLU 5 GLN 6 ASP 7 THR 8 PRO 9 TRP 10 ILE 11 LEU 12 SER 13 THR 14 GLY 15 HIS 16 ILE 17 SER 18 THR 19 GLN 20 LYS 21 ARG 22 GLN 23 ASP 24 GLY 25 GLN 26 GLN 27 THR 28 PRO 29 LYS 30 LEU 31 GLU 32 HIS 33 ARG 34 ASN 35 SER 36 THR 37 ARG 38 LEU 39 MET 40 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GB ABD77684 "PB1-F2 protein [Influenza A virus (A/Puerto Rico/8/1934(H1N1))]" 100.00 87 97.50 100.00 4.29e-19 GB ABN59421 "PB1-F2 protein [Influenza A virus (A/Alaska/1935(H1N1))]" 100.00 87 97.50 100.00 4.29e-19 GB ABO21707 "PB1-F2 protein [Influenza A virus (A/Puerto Rico/8/1934(H1N1))]" 100.00 87 97.50 100.00 4.29e-19 GB ACF41843 "PB1-F2 protein [Influenza A virus (A/Puerto Rico/8/1934(H1N1))]" 100.00 87 97.50 100.00 4.29e-19 GB ACF54596 "PB1-F2 protein [Influenza A virus (A/NYMC X-163(NYMC X-157-St. Petersburg/8/2006)(H1N1))]" 100.00 87 97.50 100.00 3.65e-19 REF YP_418248 "PB1-F2 protein [Influenza A virus (A/Puerto Rico/8/34(H1N1))]" 100.00 87 100.00 100.00 1.53e-19 SP B4URE5 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Nymc X-163(Nymc X-157-St. Petersburg/8/2006)(H1N1))]" 100.00 87 97.50 100.00 3.65e-19 SP P0C0U1 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))]" 100.00 87 100.00 100.00 1.53e-19 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $PB(1-40) 'Influenza A virus' 211044 Viruses 'Not applicable' 'Influenzavirus A' 'Influenza A virus' 'A/Puerto Rico/8/34(H1N1)' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $PB(1-40) 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PB(1-40) 2 mM . Trifluoroethanol 50 '% v/v' . water 50 '% v/v' . stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name XWIN-NMR _Version 3.1 loop_ _Vendor _Address _Electronic_address Bruker 'Bruker BioSpin GmbH, Silberstreifen 4, 76287 Rheinstetten, Germany' http://www.bruker-biospin.de/NMR/index.html stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600_MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_1D_1H_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H' _Sample_label $sample_1 save_ save_2D_1H,1H_COSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H COSY' _Sample_label $sample_1 save_ save_2D_1H,1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H,1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H NOESY' _Sample_label $sample_1 save_ save_1D_1H _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_1H_1H_COSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_1H_1H_TOCSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_1H_1H_NOESY _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0 0 mM pH 3 0 pH pressure 1 0 atm temperature 300 0 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label Trifuoroethanol H 1 'methylene protons' ppm 3.95 internal direct . . . 1.0 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details 'assigned 1H chemical shifts' loop_ _Software_label $software_1 stop_ loop_ _Experiment_label '1D 1H' '2D 1H,1H COSY' '2D 1H,1H TOCSY' '2D 1H,1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_referencing _Mol_system_component_name PB(1-40) _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET HA H 4.234 0 1 2 1 1 MET HB2 H 2.239 0 1 3 1 1 MET HB3 H 2.239 0 1 4 1 1 MET HG2 H 2.688 0 1 5 1 1 MET HG3 H 2.688 0 1 6 1 1 MET HE H 2.239 0 1 7 2 2 GLY H H 8.665 0 1 8 2 2 GLY HA2 H 4.006 0 2 9 2 2 GLY HA3 H 4.152 0 2 10 3 3 GLN H H 8.389 0 1 11 3 3 GLN HA H 4.408 0 1 12 3 3 GLN HB2 H 2.035 0 2 13 3 3 GLN HB3 H 2.189 0 2 14 3 3 GLN HG2 H 2.407 0 1 15 3 3 GLN HG3 H 2.407 0 1 16 3 3 GLN HE21 H 6.673 0 2 17 3 3 GLN HE22 H 7.372 0 2 18 4 4 GLU H H 8.432 0 1 19 4 4 GLU HA H 4.337 0 1 20 4 4 GLU HB2 H 2.089 0 2 21 4 4 GLU HB3 H 2.176 0 2 22 4 4 GLU HG2 H 2.535 0 1 23 4 4 GLU HG3 H 2.535 0 1 24 5 5 GLN H H 8.438 0 1 25 5 5 GLN HA H 4.395 0 1 26 5 5 GLN HB2 H 2.087 0 2 27 5 5 GLN HB3 H 2.195 0 2 28 5 5 GLN HG2 H 2.423 0 1 29 5 5 GLN HG3 H 2.423 0 1 30 5 5 GLN HE21 H 6.682 0 2 31 5 5 GLN HE22 H 7.379 0 2 32 6 6 ASP H H 8.279 0 1 33 6 6 ASP HA H 4.908 0 1 34 6 6 ASP HB2 H 2.968 0 2 35 6 6 ASP HB3 H 3.017 0 2 36 7 7 THR H H 7.91 0 1 37 7 7 THR HA H 4.29 0 1 38 7 7 THR HB H 4.126 0 1 39 7 7 THR HG2 H 1.249 0 1 40 8 8 PRO HA H 4.348 0 1 41 8 8 PRO HB2 H 1.87 0 2 42 8 8 PRO HB3 H 2.33 0 2 43 8 8 PRO HG2 H 2.007 0 2 44 8 8 PRO HG3 H 2.083 0 2 45 8 8 PRO HD2 H 3.72 0 2 46 8 8 PRO HD3 H 3.764 0 2 47 9 9 TRP H H 7.595 0 1 48 9 9 TRP HA H 4.465 0 1 49 9 9 TRP HB2 H 3.412 0 1 50 9 9 TRP HB3 H 3.412 0 1 51 9 9 TRP HD1 H 7.255 0 1 52 9 9 TRP HE1 H 9.828 0 1 53 9 9 TRP HE3 H 7.557 0 1 54 9 9 TRP HZ2 H 7.487 0 1 55 9 9 TRP HZ3 H 7.142 0 1 56 9 9 TRP HH2 H 7.237 0 1 57 10 10 ILE H H 7.689 0 1 58 10 10 ILE HA H 3.729 0 1 59 10 10 ILE HB H 1.881 0 1 60 10 10 ILE HG12 H 1.211 0 2 61 10 10 ILE HG13 H 1.605 0 2 62 10 10 ILE HG2 H 0.899 0 1 63 10 10 ILE HD1 H 0.899 0 1 64 11 11 LEU H H 7.998 0 1 65 11 11 LEU HA H 4.23 0 1 66 11 11 LEU HB2 H 1.75 0 2 67 11 11 LEU HB3 H 1.818 0 2 68 11 11 LEU HG H 1.573 0 1 69 11 11 LEU HD1 H 0.902 0 1 70 11 11 LEU HD2 H 0.902 0 1 71 12 12 SER H H 8.05 0 1 72 12 12 SER HA H 4.349 0 1 73 12 12 SER HB2 H 3.961 0 2 74 12 12 SER HB3 H 4.046 0 2 75 13 13 THR H H 7.969 0 1 76 13 13 THR HA H 4.091 0 1 77 13 13 THR HB H 4.151 0 1 78 13 13 THR HG2 H 1.099 0 1 79 14 14 GLY H H 8.273 0 1 80 14 14 GLY HA2 H 3.924 0 1 81 14 14 GLY HA3 H 3.924 0 1 82 15 15 HIS H H 8.173 0 1 83 15 15 HIS HA H 4.604 0 1 84 15 15 HIS HB2 H 3.318 0 2 85 15 15 HIS HB3 H 3.403 0 2 86 15 15 HIS HD2 H 7.309 0 1 87 15 15 HIS HE1 H 8.541 0 1 88 16 16 ILE H H 8.168 0 1 89 16 16 ILE HA H 3.997 0 1 90 16 16 ILE HB H 1.944 0 1 91 16 16 ILE HG12 H 1.233 0 2 92 16 16 ILE HG13 H 1.655 0 2 93 16 16 ILE HG2 H 0.968 0 1 94 16 16 ILE HD1 H 0.895 0 1 95 17 17 SER H H 8.269 0 1 96 17 17 SER HA H 4.35 0 1 97 17 17 SER HB2 H 3.972 0 2 98 17 17 SER HB3 H 4.046 0 2 99 18 18 THR H H 7.945 0 1 100 18 18 THR HA H 4.195 0 1 101 18 18 THR HB H 4.364 0 1 102 18 18 THR HG2 H 1.294 0 1 103 19 19 GLN H H 8.056 0 1 104 19 19 GLN HA H 4.219 0 1 105 19 19 GLN HB2 H 2.126 0 2 106 19 19 GLN HB3 H 2.183 0 2 107 19 19 GLN HG2 H 2.412 0 1 108 19 19 GLN HG3 H 2.412 0 1 109 19 19 GLN HE21 H 6.671 0 2 110 19 19 GLN HE22 H 7.32 0 2 111 20 20 LYS H H 8.232 0 1 112 20 20 LYS HA H 4.243 0 1 113 20 20 LYS HB2 H 1.919 0 1 114 20 20 LYS HB3 H 1.919 0 1 115 20 20 LYS HG2 H 1.506 0 2 116 20 20 LYS HG3 H 1.593 0 2 117 20 20 LYS HD2 H 1.736 0 1 118 20 20 LYS HD3 H 1.736 0 1 119 20 20 LYS HE2 H 3.023 0 1 120 20 20 LYS HE3 H 3.023 0 1 121 20 20 LYS HZ H 7.603 0 1 122 21 21 ARG H H 8.027 0 1 123 21 21 ARG HA H 4.288 0 1 124 21 21 ARG HB2 H 1.93 0 2 125 21 21 ARG HB3 H 1.965 0 2 126 21 21 ARG HG2 H 1.692 0 2 127 21 21 ARG HG3 H 1.792 0 2 128 21 21 ARG HD2 H 3.248 0 1 129 21 21 ARG HD3 H 3.248 0 1 130 21 21 ARG HE H 7.204 0 1 131 22 22 GLN H H 8.255 0 1 132 22 22 GLN HA H 4.324 0 1 133 22 22 GLN HB2 H 2.126 0 2 134 22 22 GLN HB3 H 2.195 0 2 135 22 22 GLN HG2 H 2.44 0 2 136 22 22 GLN HG3 H 2.481 0 2 137 22 22 GLN HE21 H 6.647 0 2 138 22 22 GLN HE22 H 7.326 0 2 139 23 23 ASP H H 8.372 0 1 140 23 23 ASP HA H 4.757 0 1 141 23 23 ASP HB2 H 2.993 0 1 142 23 23 ASP HB3 H 2.993 0 1 143 24 24 GLY H H 8.267 0 1 144 24 24 GLY HA2 H 4.026 0 1 145 24 24 GLY HA3 H 4.026 0 1 146 25 25 GLN H H 8.082 0 1 147 25 25 GLN HA H 4.387 0 1 148 25 25 GLN HB2 H 2.111 0 2 149 25 25 GLN HB3 H 2.218 0 2 150 25 25 GLN HG2 H 2.446 0 1 151 25 25 GLN HG3 H 2.446 0 1 152 25 25 GLN HE21 H 6.692 0 2 153 25 25 GLN HE22 H 7.387 0 2 154 26 26 GLN H H 8.29 0 1 155 26 26 GLN HA H 4.488 0 1 156 26 26 GLN HB2 H 2.065 0 2 157 26 26 GLN HB3 H 2.22 0 2 158 26 26 GLN HG2 H 2.447 0 1 159 26 26 GLN HG3 H 2.447 0 1 160 26 26 GLN HE21 H 6.684 0 2 161 26 26 GLN HE22 H 7.439 0 2 162 27 27 THR H H 7.951 0 1 163 27 27 THR HA H 4.57 0 1 164 27 27 THR HB H 4.312 0 1 165 27 27 THR HG2 H 1.307 0 1 166 28 28 PRO HA H 4.442 0 1 167 28 28 PRO HB2 H 1.926 0 2 168 28 28 PRO HB3 H 2.364 0 2 169 28 28 PRO HG2 H 2.043 0 2 170 28 28 PRO HG3 H 2.114 0 2 171 28 28 PRO HD2 H 3.738 0 2 172 28 28 PRO HD3 H 3.859 0 2 173 29 29 LYS H H 7.984 0 1 174 29 29 LYS HA H 4.278 0 1 175 29 29 LYS HB2 H 1.887 0 1 176 29 29 LYS HB3 H 1.887 0 1 177 29 29 LYS HG2 H 1.479 0 2 178 29 29 LYS HG3 H 1.575 0 2 179 29 29 LYS HD2 H 1.755 0 1 180 29 29 LYS HD3 H 1.755 0 1 181 29 29 LYS HE2 H 3.049 0 1 182 29 29 LYS HE3 H 3.049 0 1 183 29 29 LYS HZ H 7.603 0 1 184 30 30 LEU H H 7.84 0 1 185 30 30 LEU HA H 4.324 0 1 186 30 30 LEU HB2 H 1.658 0 2 187 30 30 LEU HB3 H 1.731 0 2 188 30 30 LEU HG H 1.658 0 1 189 30 30 LEU HD1 H 0.921 0 2 190 30 30 LEU HD2 H 0.971 0 2 191 31 31 GLU H H 8.056 0 1 192 31 31 GLU HA H 4.324 0 1 193 31 31 GLU HB2 H 2.11 0 2 194 31 31 GLU HB3 H 2.203 0 2 195 31 31 GLU HG2 H 2.47 0 1 196 31 31 GLU HG3 H 2.47 0 1 197 32 32 HIS H H 8.29 0 1 198 32 32 HIS HA H 4.686 0 1 199 32 32 HIS HB2 H 3.259 0 2 200 32 32 HIS HB3 H 3.38 0 2 201 32 32 HIS HD2 H 7.343 0 1 202 32 32 HIS HE1 H 8.588 0 1 203 33 33 ARG H H 8.262 0 1 204 33 33 ARG HA H 4.351 0 1 205 33 33 ARG HB2 H 1.883 0 2 206 33 33 ARG HB3 H 1.955 0 2 207 33 33 ARG HG2 H 1.69 0 2 208 33 33 ARG HG3 H 1.736 0 2 209 33 33 ARG HD2 H 3.237 0 1 210 33 33 ARG HD3 H 3.237 0 1 211 33 33 ARG HE H 7.194 0 1 212 34 34 ASN H H 8.466 0 1 213 34 34 ASN HA H 4.779 0 1 214 34 34 ASN HB2 H 2.866 0 2 215 34 34 ASN HB3 H 2.908 0 2 216 34 34 ASN HD21 H 6.771 0 2 217 34 34 ASN HD22 H 7.507 0 2 218 35 35 SER H H 8.214 0 1 219 35 35 SER HA H 4.511 0 1 220 35 35 SER HB2 H 3.936 0 2 221 35 35 SER HB3 H 4.034 0 2 222 36 36 THR H H 8.028 0 1 223 36 36 THR HA H 4.337 0 1 224 36 36 THR HB H 4.337 0 1 225 36 36 THR HG2 H 1.274 0 1 226 37 37 ARG H H 8.074 0 1 227 37 37 ARG HA H 4.361 0 1 228 37 37 ARG HB2 H 1.846 0 2 229 37 37 ARG HB3 H 1.942 0 2 230 37 37 ARG HG2 H 1.702 0 1 231 37 37 ARG HG3 H 1.702 0 1 232 37 37 ARG HD2 H 3.237 0 1 233 37 37 ARG HD3 H 3.237 0 1 234 37 37 ARG HE H 7.187 0 1 235 38 38 LEU H H 7.957 0 1 236 38 38 LEU HA H 4.359 0 1 237 38 38 LEU HB2 H 1.729 0 1 238 38 38 LEU HB3 H 1.729 0 1 239 38 38 LEU HG H 1.67 0 1 240 38 38 LEU HD1 H 0.922 0 2 241 38 38 LEU HD2 H 0.972 0 2 242 39 39 MET H H 8.004 0 1 243 39 39 MET HA H 4.5 0 1 244 39 39 MET HB2 H 2.088 0 2 245 39 39 MET HB3 H 2.194 0 2 246 39 39 MET HG2 H 2.586 0 2 247 39 39 MET HG3 H 2.646 0 2 248 39 39 MET HE H 2.194 0 1 249 40 40 GLY H H 8.08 0 1 250 40 40 GLY HA2 H 3.925 0 2 251 40 40 GLY HA3 H 3.987 0 2 stop_ save_