data_7290 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein ; _BMRB_accession_number 7290 _BMRB_flat_file_name bmr7290.str _Entry_type original _Submission_date 2006-09-06 _Accession_date 2006-09-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Assigned 1H chemical shifts of the central fragment PB(30-70) of the PB1-F2 protein' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bruns Karsten . . 2 Studtrucker Nicole . . 3 Sharma Alok . . 4 Fossen Torgils . . 5 Mitzner David . . 6 Eissmann Andre . . 7 Tessmer Uwe . . 8 Henklein Peter . . 9 Wray Victor . . 10 Schubert Ulrich . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 291 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-01-08 update author 'update the entry citation' 2006-10-31 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 7258 'NMR data of the C-terminal fragment PB(50-87) of the PB1-F2 protein' 7289 'NMR data of the N-terminal fragment PB(1-40) of the PB1-F2 protein' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17052982 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bruns Karsten . . 2 Studtrucker Nicole . . 3 Sharma Alok . . 4 Fossen Torgils . . 5 Mitzner David . . 6 Eissmann Andre . . 7 Tessmer Uwe . . 8 Roder R. . . 9 Henklein Peter . . 10 Wray Victor . . 11 Schubert Ulrich . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 282 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 353 _Page_last 363 _Year 2006 _Details . loop_ _Keyword 'Influenza A virus' PB1-F2 'Structure and oligomerization' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name PB(30-70) _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label PB(30-70) $PB(30-70) stop_ _System_molecular_weight 4978 _System_physical_state native _System_oligomer_state protein _System_paramagnetic no _System_thiol_state unknown _Database_query_date . _Details 'Central fragment of influenza A virus PB1-F2 protein' save_ ######################## # Monomeric polymers # ######################## save_PB(30-70) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common PB(30-70) _Molecular_mass 4978 _Mol_thiol_state unknown _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 41 _Mol_residue_sequence ; LEHRNSTRLMGHCQKTMNQV VMPKQIVYWKQWLSLRNPIL V ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 30 LEU 2 31 GLU 3 32 HIS 4 33 ARG 5 34 ASN 6 35 SER 7 36 THR 8 37 ARG 9 38 LEU 10 39 MET 11 40 GLY 12 41 HIS 13 42 CYS 14 43 GLN 15 44 LYS 16 45 THR 17 46 MET 18 47 ASN 19 48 GLN 20 49 VAL 21 50 VAL 22 51 MET 23 52 PRO 24 53 LYS 25 54 GLN 26 55 ILE 27 56 VAL 28 57 TYR 29 58 TRP 30 59 LYS 31 60 GLN 32 61 TRP 33 62 LEU 34 63 SER 35 64 LEU 36 65 ARG 37 66 ASN 38 67 PRO 39 68 ILE 40 69 LEU 41 70 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 7258 PB1-F2 51.22 38 100.00 100.00 4.82e-05 PDB 2HN8 "Structural Characterization And Oligomerization Of Pb1-F2, A Pro-Apoptotic Influenza A Virus Protein" 51.22 38 100.00 100.00 4.82e-05 GB ABD62790 "PB1-F2 protein [Influenza A virus (A/Melbourne/1935(H1N1))]" 100.00 90 100.00 100.00 1.17e-20 GB ABD77805 "PB1-F2 protein [Influenza A virus (A/Wilson-Smith/1933(H1N1))]" 100.00 90 97.56 97.56 1.08e-19 GB ABF47964 "PB1-F2 protein [Influenza A virus (A/WSN/1933 TS61(H1N1))]" 100.00 90 97.56 97.56 1.29e-19 GB ABM22200 "PB1-F2 protein [Influenza A virus (A/Memphis/42/1983(H1N1))]" 68.29 57 100.00 100.00 6.02e-11 GB ABO38063 "PB1-F2 protein [Influenza A virus (A/AA/Marton/1943(H1N1))]" 100.00 90 97.56 97.56 1.25e-19 REF YP_418248 "PB1-F2 protein [Influenza A virus (A/Puerto Rico/8/34(H1N1))]" 100.00 87 100.00 100.00 1.10e-20 SP A4GCJ5 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Henry/1936(H1N1))]" 100.00 90 97.56 97.56 3.15e-19 SP A4GCL7 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Iowa/1943(H1N1))]" 100.00 90 97.56 97.56 1.25e-19 SP A4GCM8 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Phila/1935(H1N1))]" 100.00 90 100.00 100.00 1.29e-20 SP P0C0U1 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))]" 100.00 87 100.00 100.00 1.10e-20 SP Q20MH0 "RecName: Full=Protein PB1-F2 [Influenza A virus (A/Wilson-Smith/1933(H1N1))]" 100.00 90 97.56 97.56 1.08e-19 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $PB(30-70) 'Influenza A virus' 211044 Viruses 'Not applicable' 'Influenzavirus A' 'Influenza A virus' 'A/Puerto Rico/8/34(H1N1)' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $PB(30-70) 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PB(30-70) 2 mM . Trifluoroethanol 50 '% v/v' . water 50 '% v/v' . stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name XWIN-NMR _Version 3.1 loop_ _Vendor _Address _Electronic_address Bruker 'Bruker BioSpin GmbH, Silberstreifen 4, 76287 Rheinstetten, Germany' http://www.bruker-biospin.de/NMR/index.html stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600_MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_1D_1H_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H' _Sample_label $sample_1 save_ save_2D_1H,1H_COSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H COSY' _Sample_label $sample_1 save_ save_2D_1H,1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H,1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H NOESY' _Sample_label $sample_1 save_ save_1D_1H _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_1H_1H_COSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_1H_1H_TOCSY _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_2D_1H_1H_NOESY _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H,1H NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0 0 mM pH 3 0 pH pressure 1 0 atm temperature 300 0 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label Trifluoroethanol H 1 'methylene protons' ppm 3.95 internal direct . . . 1.0 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details 'assigned 1H chemical shifts' loop_ _Software_label $software_1 stop_ loop_ _Experiment_label '1D 1H' '2D 1H,1H COSY' '2D 1H,1H TOCSY' '2D 1H,1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_referencing _Mol_system_component_name PB(30-70) _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 30 1 LEU HA H 4.047 0 1 2 30 1 LEU HB2 H 1.71 0 2 3 30 1 LEU HB3 H 1.746 0 2 4 30 1 LEU HG H 1.645 0 1 5 30 1 LEU HD1 H 0.897 0 2 6 30 1 LEU HD2 H 0.972 0 2 7 31 2 GLU H H 8.591 0 1 8 31 2 GLU HA H 4.459 0 1 9 31 2 GLU HB2 H 2.025 0 2 10 31 2 GLU HB3 H 2.11 0 2 11 31 2 GLU HG2 H 2.478 0 1 12 31 2 GLU HG3 H 2.478 0 1 13 32 3 HIS H H 8.592 0 1 14 32 3 HIS HA H 4.766 0 1 15 32 3 HIS HB2 H 3.228 0 2 16 32 3 HIS HB3 H 3.322 0 2 17 32 3 HIS HD2 H 7.349 0 1 18 32 3 HIS HE1 H 8.605 0 1 19 33 4 ARG H H 8.411 0 1 20 33 4 ARG HA H 4.403 0 1 21 33 4 ARG HB2 H 1.818 0 2 22 33 4 ARG HB3 H 1.904 0 2 23 33 4 ARG HG2 H 1.69 0 1 24 33 4 ARG HG3 H 1.69 0 1 25 33 4 ARG HD2 H 3.241 0 1 26 33 4 ARG HD3 H 3.241 0 1 27 33 4 ARG HE H 7.196 0 1 28 33 4 ARG HH11 H 6.64 0 1 29 33 4 ARG HH12 H 6.64 0 1 30 33 4 ARG HH21 H 6.64 0 1 31 33 4 ARG HH22 H 6.64 0 1 32 34 5 ASN H H 8.534 0 1 33 34 5 ASN HA H 4.825 0 1 34 34 5 ASN HB2 H 2.852 0 2 35 34 5 ASN HB3 H 2.914 0 2 36 34 5 ASN HD21 H 6.83 0 2 37 34 5 ASN HD22 H 7.547 0 2 38 35 6 SER H H 8.314 0 1 39 35 6 SER HA H 4.528 0 1 40 35 6 SER HB2 H 3.921 0 2 41 35 6 SER HB3 H 4.039 0 2 42 36 7 THR H H 8.142 0 1 43 36 7 THR HA H 4.31 0 1 44 36 7 THR HB H 4.31 0 1 45 36 7 THR HG2 H 1.296 0 1 46 37 8 ARG H H 8.133 0 1 47 37 8 ARG HA H 4.321 0 1 48 37 8 ARG HB2 H 1.853 0 2 49 37 8 ARG HB3 H 1.912 0 2 50 37 8 ARG HG2 H 1.7 0 1 51 37 8 ARG HG3 H 1.7 0 1 52 37 8 ARG HD2 H 3.251 0 1 53 37 8 ARG HD3 H 3.251 0 1 54 37 8 ARG HE H 7.2 0 1 55 37 8 ARG HH11 H 6.695 0 1 56 37 8 ARG HH12 H 6.695 0 1 57 37 8 ARG HH21 H 6.695 0 1 58 37 8 ARG HH22 H 6.695 0 1 59 38 9 LEU H H 7.925 0 1 60 38 9 LEU HA H 4.345 0 1 61 38 9 LEU HB2 H 1.735 0 1 62 38 9 LEU HB3 H 1.735 0 1 63 38 9 LEU HG H 1.676 0 1 64 38 9 LEU HD1 H 0.9 0 2 65 38 9 LEU HD2 H 0.952 0 2 66 39 10 MET H H 8.04 0 1 67 39 10 MET HA H 4.441 0 1 68 39 10 MET HB2 H 2.096 0 2 69 39 10 MET HB3 H 2.174 0 2 70 39 10 MET HG2 H 2.6 0 2 71 39 10 MET HG3 H 2.675 0 2 72 39 10 MET HE H 2.142 0 1 73 40 11 GLY H H 8.25 0 1 74 40 11 GLY HA2 H 3.956 0 1 75 40 11 GLY HA3 H 3.956 0 1 76 41 12 HIS H H 8.238 0 1 77 41 12 HIS HA H 4.721 0 1 78 41 12 HIS HB2 H 3.268 0 2 79 41 12 HIS HB3 H 3.424 0 2 80 41 12 HIS HD2 H 7.331 0 1 81 41 12 HIS HE1 H 8.59 0 1 82 42 13 CYS H H 8.331 0 1 83 42 13 CYS HA H 4.476 0 1 84 42 13 CYS HB2 H 3.024 0 1 85 42 13 CYS HB3 H 3.024 0 1 86 43 14 GLN H H 8.481 0 1 87 43 14 GLN HA H 4.311 0 1 88 43 14 GLN HB2 H 2.099 0 2 89 43 14 GLN HB3 H 2.181 0 2 90 43 14 GLN HG2 H 2.45 0 1 91 43 14 GLN HG3 H 2.45 0 1 92 44 15 LYS H H 8.201 0 1 93 44 15 LYS HA H 4.369 0 1 94 44 15 LYS HB2 H 1.857 0 2 95 44 15 LYS HB3 H 1.914 0 2 96 44 15 LYS HG2 H 1.495 0 2 97 44 15 LYS HG3 H 1.54 0 2 98 44 15 LYS HD2 H 1.736 0 1 99 44 15 LYS HD3 H 1.736 0 1 100 44 15 LYS HE2 H 3.038 0 1 101 44 15 LYS HE3 H 3.038 0 1 102 44 15 LYS HZ H 7.618 0 1 103 45 16 THR H H 7.905 0 1 104 45 16 THR HA H 4.333 0 1 105 45 16 THR HB H 4.333 0 1 106 45 16 THR HG2 H 1.248 0 1 107 46 17 MET H H 8.243 0 1 108 46 17 MET HA H 4.485 0 1 109 46 17 MET HB2 H 2.11 0 2 110 46 17 MET HB3 H 2.155 0 2 111 46 17 MET HG2 H 2.608 0 2 112 46 17 MET HG3 H 2.656 0 2 113 46 17 MET HE H 2.128 0 1 114 47 18 ASN H H 8.225 0 1 115 47 18 ASN HA H 4.65 0 1 116 47 18 ASN HB2 H 2.839 0 1 117 47 18 ASN HB3 H 2.839 0 1 118 47 18 ASN HD21 H 6.706 0 2 119 47 18 ASN HD22 H 7.442 0 2 120 48 19 GLN H H 8.116 0 1 121 48 19 GLN HA H 4.363 0 1 122 48 19 GLN HB2 H 2.146 0 2 123 48 19 GLN HB3 H 2.186 0 2 124 48 19 GLN HG2 H 2.425 0 1 125 48 19 GLN HG3 H 2.425 0 1 126 48 19 GLN HE21 H 6.65 0 2 127 48 19 GLN HE22 H 7.363 0 2 128 49 20 VAL H H 7.884 0 1 129 49 20 VAL HA H 4.113 0 1 130 49 20 VAL HB H 2.184 0 1 131 49 20 VAL HG1 H 0.897 0 2 132 49 20 VAL HG2 H 0.957 0 2 133 50 21 VAL H H 7.816 0 1 134 50 21 VAL HA H 4.176 0 1 135 50 21 VAL HB H 2.14 0 1 136 50 21 VAL HG1 H 0.981 0 1 137 50 21 VAL HG2 H 0.981 0 1 138 51 22 MET H H 8.08 0 1 139 51 22 MET HA H 4.814 0 1 140 51 22 MET HB2 H 2.109 0 1 141 51 22 MET HB3 H 2.109 0 1 142 51 22 MET HG2 H 2.608 0 2 143 51 22 MET HG3 H 2.671 0 2 144 51 22 MET HE H 2.109 0 1 145 52 23 PRO HA H 4.409 0 1 146 52 23 PRO HB2 H 1.967 0 2 147 52 23 PRO HB3 H 2.338 0 2 148 52 23 PRO HG2 H 2.033 0 1 149 52 23 PRO HG3 H 2.033 0 1 150 52 23 PRO HD2 H 3.779 0 1 151 52 23 PRO HD3 H 3.779 0 1 152 53 24 LYS H H 7.927 0 1 153 53 24 LYS HA H 4.045 0 1 154 53 24 LYS HB2 H 1.736 0 2 155 53 24 LYS HB3 H 1.885 0 2 156 53 24 LYS HG2 H 1.482 0 2 157 53 24 LYS HG3 H 1.567 0 2 158 53 24 LYS HD2 H 1.697 0 1 159 53 24 LYS HD3 H 1.697 0 1 160 53 24 LYS HE2 H 3.026 0 1 161 53 24 LYS HE3 H 3.026 0 1 162 53 24 LYS HZ H 7.622 0 1 163 54 25 GLN H H 8.377 0 1 164 54 25 GLN HA H 4.116 0 1 165 54 25 GLN HB2 H 2.117 0 2 166 54 25 GLN HB3 H 2.197 0 2 167 54 25 GLN HG2 H 2.318 0 2 168 54 25 GLN HG3 H 2.393 0 2 169 54 25 GLN HE21 H 6.578 0 2 170 54 25 GLN HE22 H 6.964 0 2 171 55 26 ILE H H 7.637 0 1 172 55 26 ILE HA H 4.117 0 1 173 55 26 ILE HB H 2.072 0 1 174 55 26 ILE HG12 H 1.316 0 2 175 55 26 ILE HG13 H 1.674 0 2 176 55 26 ILE HG2 H 1.046 0 1 177 55 26 ILE HD1 H 0.935 0 1 178 56 27 VAL H H 7.635 0 1 179 56 27 VAL HA H 3.692 0 1 180 56 27 VAL HB H 2.149 0 1 181 56 27 VAL HG1 H 0.936 0 2 182 56 27 VAL HG2 H 1.045 0 2 183 57 28 TYR H H 7.97 0 1 184 57 28 TYR HA H 4.299 0 1 185 57 28 TYR HB2 H 3.075 0 1 186 57 28 TYR HB3 H 3.075 0 1 187 57 28 TYR HD1 H 6.848 0 1 188 57 28 TYR HD2 H 6.848 0 1 189 57 28 TYR HE1 H 6.534 0 1 190 57 28 TYR HE2 H 6.534 0 1 191 58 29 TRP H H 8.249 0 1 192 58 29 TRP HA H 4.65 0 1 193 58 29 TRP HB2 H 3.488 0 1 194 58 29 TRP HB3 H 3.488 0 1 195 58 29 TRP HD1 H 7.269 0 1 196 58 29 TRP HE1 H 9.637 0 1 197 58 29 TRP HE3 H 7.596 0 1 198 58 29 TRP HZ2 H 6.674 0 1 199 58 29 TRP HZ3 H 7.436 0 1 200 58 29 TRP HH2 H 7.099 0 1 201 59 30 LYS H H 8.586 0 1 202 59 30 LYS HA H 3.996 0 1 203 59 30 LYS HB2 H 1.954 0 2 204 59 30 LYS HB3 H 2.034 0 2 205 59 30 LYS HG2 H 1.484 0 1 206 59 30 LYS HG3 H 1.484 0 1 207 59 30 LYS HD2 H 1.69 0 1 208 59 30 LYS HD3 H 1.69 0 1 209 59 30 LYS HE2 H 2.996 0 1 210 59 30 LYS HE3 H 2.996 0 1 211 59 30 LYS HZ H 7.606 0 1 212 60 31 GLN H H 8.312 0 1 213 60 31 GLN HA H 4.042 0 1 214 60 31 GLN HB2 H 2.295 0 1 215 60 31 GLN HB3 H 2.295 0 1 216 60 31 GLN HG2 H 2.499 0 1 217 60 31 GLN HG3 H 2.499 0 1 218 60 31 GLN HE21 H 6.453 0 2 219 60 31 GLN HE22 H 6.828 0 2 220 61 32 TRP H H 8.493 0 1 221 61 32 TRP HA H 4.194 0 1 222 61 32 TRP HB2 H 3.112 0 2 223 61 32 TRP HB3 H 3.481 0 2 224 61 32 TRP HD1 H 7.023 0 1 225 61 32 TRP HE1 H 9.607 0 1 226 61 32 TRP HE3 H 7.482 0 1 227 61 32 TRP HZ2 H 7.45 0 1 228 61 32 TRP HZ3 H 7.081 0 1 229 61 32 TRP HH2 H 7.21 0 1 230 62 33 LEU H H 8.642 0 1 231 62 33 LEU HA H 3.902 0 1 232 62 33 LEU HB2 H 1.864 0 1 233 62 33 LEU HB3 H 1.864 0 1 234 62 33 LEU HG H 1.496 0 1 235 62 33 LEU HD1 H 0.849 0 2 236 62 33 LEU HD2 H 0.918 0 2 237 63 34 SER H H 7.878 0 1 238 63 34 SER HA H 4.247 0 1 239 63 34 SER HB2 H 3.944 0 2 240 63 34 SER HB3 H 4.045 0 2 241 64 35 LEU H H 7.492 0 1 242 64 35 LEU HA H 4.228 0 1 243 64 35 LEU HB2 H 1.684 0 2 244 64 35 LEU HB3 H 1.784 0 2 245 64 35 LEU HG H 1.5 0 1 246 64 35 LEU HD1 H 0.857 0 1 247 64 35 LEU HD2 H 0.857 0 1 248 65 36 ARG H H 7.498 0 1 249 65 36 ARG HA H 4.135 0 1 250 65 36 ARG HB2 H 1.571 0 2 251 65 36 ARG HB3 H 1.728 0 2 252 65 36 ARG HG2 H 1.396 0 1 253 65 36 ARG HG3 H 1.396 0 1 254 65 36 ARG HD2 H 2.771 0 1 255 65 36 ARG HD3 H 2.771 0 1 256 65 36 ARG HH11 H 6.83 0 1 257 65 36 ARG HH12 H 6.83 0 1 258 65 36 ARG HH21 H 6.83 0 1 259 65 36 ARG HH22 H 6.83 0 1 260 66 37 ASN H H 7.718 0 1 261 66 37 ASN HA H 4.942 0 1 262 66 37 ASN HB2 H 2.728 0 2 263 66 37 ASN HB3 H 2.866 0 2 264 66 37 ASN HD21 H 7.359 0 2 265 66 37 ASN HD22 H 6.323 0 2 266 67 38 PRO HA H 4.405 0 1 267 67 38 PRO HB2 H 1.984 0 2 268 67 38 PRO HB3 H 2.401 0 2 269 67 38 PRO HG2 H 2.053 0 2 270 67 38 PRO HG3 H 2.167 0 2 271 67 38 PRO HD2 H 3.698 0 2 272 67 38 PRO HD3 H 3.904 0 2 273 68 39 ILE H H 7.79 0 1 274 68 39 ILE HA H 4.054 0 1 275 68 39 ILE HB H 1.932 0 1 276 68 39 ILE HG12 H 1.256 0 2 277 68 39 ILE HG13 H 1.536 0 2 278 68 39 ILE HG2 H 0.924 0 1 279 68 39 ILE HD1 H 0.924 0 1 280 69 40 LEU H H 7.605 0 1 281 69 40 LEU HA H 4.346 0 1 282 69 40 LEU HB2 H 1.732 0 1 283 69 40 LEU HB3 H 1.732 0 1 284 69 40 LEU HG H 1.641 0 1 285 69 40 LEU HD1 H 0.892 0 2 286 69 40 LEU HD2 H 0.973 0 2 287 70 41 VAL H H 7.515 0 1 288 70 41 VAL HA H 4.1 0 1 289 70 41 VAL HB H 2.151 0 1 290 70 41 VAL HG1 H 0.971 0 1 291 70 41 VAL HG2 H 0.971 0 1 stop_ save_