BMRB Entry 11580
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11580
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Title: NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion
Deposition date: 2014-11-05 Original release date: 2016-07-13
Authors: Mahajan, Mukesh; Bhattacharjya, Surajit
Citation: Mahajan, Mukesh; Bhattacharjya, Surajit. "NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion" Biochim. Biophys. Acta ., .-..
Assembly members:
Fusion_Peptide, polymer, 19 residues, 2280.706 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Fusion_Peptide: MWKTPTLKYFGGFNFSQIL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 17 |
| 1H chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fusion peptide | 1 |
Entities:
Entity 1, Fusion peptide 19 residues - 2280.706 Da.
| 1 | MET | TRP | LYS | THR | PRO | THR | LEU | LYS | TYR | PHE | ||||
| 2 | GLY | GLY | PHE | ASN | PHE | SER | GLN | ILE | LEU |
Samples:
sample_1: DPC, [U-99% 2H], 125 mM; Fusion Peptide 0.5 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 4.7; temperature: 315 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | anisotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Structure calculation
SPARKY v3.113, Goddard - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz