BMRB Entry 7290
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7290
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Title: Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein PubMed: 17052982
Deposition date: 2006-09-06 Original release date: 2006-10-31
Authors: Bruns, Karsten; Studtrucker, Nicole; Sharma, Alok; Fossen, Torgils; Mitzner, David; Eissmann, Andre; Tessmer, Uwe; Henklein, Peter; Wray, Victor; Schubert, Ulrich
Citation: Bruns, Karsten; Studtrucker, Nicole; Sharma, Alok; Fossen, Torgils; Mitzner, David; Eissmann, Andre; Tessmer, Uwe; Roder, R.; Henklein, Peter; Wray, Victor; Schubert, Ulrich. "Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein" J. Biol. Chem. 282, 353-363 (2006).
Assembly members:
PB(30-70), polymer, 41 residues, 4978 Da.
Natural source: Common Name: Influenza A virus Taxonomy ID: 211044 Superkingdom: Viruses Kingdom: Not applicable Genus/species: Influenzavirus A Influenza A virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
PB(30-70): LEHRNSTRLMGHCQKTMNQV
VMPKQIVYWKQWLSLRNPIL
V
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 351 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PB(30-70) | 1 |
Entities:
Entity 1, PB(30-70) 41 residues - 4978 Da.
Central fragment PB(30-70) of the PB1-F2 protein comprising amino acid residues Leu-30 to Val-70
| 1 | LEU | GLU | HIS | ARG | ASN | SER | THR | ARG | LEU | MET | ||||
| 2 | GLY | HIS | CYS | GLN | LYS | THR | MET | ASN | GLN | VAL | ||||
| 3 | VAL | MET | PRO | LYS | GLN | ILE | VAL | TYR | TRP | LYS | ||||
| 4 | GLN | TRP | LEU | SER | LEU | ARG | ASN | PRO | ILE | LEU | ||||
| 5 | VAL |
Samples:
sample_1: PB(30-70) 2 mM; Trifluoroethanol 50 % v/v; water 50 % v/v
conditions_1: ionic strength: 0 mM; pH: 3; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | not available | conditions_1 |
| 2D 1H,1H COSY | sample_1 | not available | conditions_1 |
| 2D 1H,1H TOCSY | sample_1 | not available | conditions_1 |
| 2D 1H,1H NOESY | sample_1 | not available | conditions_1 |
Software:
XWIN-NMR v3.1, Bruker -
NMR spectrometers:
- Bruker DMX 600 MHz