Sequence for Human DHFR:

             1   VAL     2   GLY     3   SER     4   LEU     5   ASN
             6   CYS     7   ILE     8   VAL     9   ALA    10   VAL
            11   SER    12   GLN    13   ASN    14   MET    15   GLY
            16   ILE    17   GLY    18   LYS    19   ASN    20   GLY
            21   ASP    22   LEU    23   PRO    24   TRP    25   PRO
            26   PRO    27   LEU    28   ARG    29   ASN    30   GLU
            31   PHE    32   ARG    33   TYR    34   PHE    35   GLN
            36   ARG    37   MET    38   THR    39   THR    40   THR
            41   SER    42   SER    43   VAL    44   GLU    45   GLY
            46   LYS    47   GLN    48   ASN    49   LEU    50   VAL
            51   ILE    52   MET    53   GLY    54   LYS    55   LYS
            56   THR    57   TRP    58   PHE    59   SER    60   ILE
            61   PRO    62   GLU    63   LYS    64   ASN    65   ARG
            66   PRO    67   LEU    68   LYS    69   GLY    70   ARG
            71   ILE    72   ASN    73   LEU    74   VAL    75   LEU
            76   SER    77   ARG    78   GLU    79   LEU    80   LYS
            81   GLU    82   PRO    83   PRO    84   GLN    85   GLY
            86   ALA    87   HIS    88   PHE    89   LEU    90   SER
            91   ARG    92   SER    93   LEU    94   ASP    95   ASP
            96   ALA    97   LEU    98   LYS    99   LEU   100   THR
           101   GLU   102   GLN   103   PRO   104   GLU   105   LEU
           106   ALA   107   ASN   108   LYS   109   VAL   110   ASP
           111   MET   112   VAL   113   TRP   114   ILE   115   VAL
           116   GLY   117   GLY   118   SER   119   SER   120   VAL
           121   TYR   122   LYS   123   GLU   124   ALA   125   MET
           126   ASN   127   HIS   128   PRO   129   GLY   130   HIS
           131   LEU   132   LYS   133   LEU   134   PHE   135   VAL
           136   THR   137   ARG   138   ILE   139   MET   140   GLN
           141   ASP   142   PHE   143   GLU   144   SER   145   ASP
           146   THR   147   PHE   148   PHE   149   PRO   150   GLU
           151   ILE   152   ASP   153   LEU   154   GLU   155   LYS
           156   TYR   157   LYS   158   LEU   159   LEU   160   PRO
           161   GLU   162   TYR   163   PRO   164   GLY   165   VAL
           166   LEU   167   SER   168   ASP   169   VAL   170   GLN
           171   GLU   172   GLU   173   LYS   174   GLY   175   ILE
           176   LYS   177   TYR   178   LYS   179   PHE   180   GLU
           181   VAL   182   TYR   183   GLU   184   LYS   185   ASN
           186   ASP

   Formula Weight for Human DHFR:  21322 Da



Human           VGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSI 60
Chicken         VRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSI 60
Drosophila      MLRFNLIVAVCENFGIGIRGDLPWR-IKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGV 59
E. coli         --MISLIAALAVDRVIGMENAMPWN-LPADLAWFKRNTLN-------KPVIMGRHTWESI 50
                   :. *.*:. :  **  . :**  :  :  :*.* *         : *:**::*: .:

Human       PEKNRPLKGRINLVLSRELKEP--PQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGS 118
Chicken     PEKNRPLKDRINIVLSRELKEA--PKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGT 118
Drosophila  PESKRPLPDRLNIVLSTTLQESDLPKG-VLLCPNLETAMKILEE---QNEVENIWIVGGS 115
E. coli     G---RPLPGRKNIILSSQPGTD----DRVTWVKSVDEAIAACGD------VPEIMVIGGG 97
                    *** .* *::**          .      .:: *:    .      *  : ::**

Human       SVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYK 178
Chicken     AVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEEDGIQYK 178
Drosophila  GVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIP-DSFREVAPDSDMPLGVQEENGIKFE 174
E. coli     RVYEQFLPK--AQKLYLTHIDAEVEGDTHFPDYEPDDWESVFS---EFHDADAQNSHSYC 152
                 **:  :      :*::*:*  ..: **.**    ..:. :        . : :.. .:

Human       FEVYEKND--- 186
Chicken     FEVYQKSVLAQ 189
Drosophila  YKILEKHS--- 182
E. coli     FEILERR---- 159
                ::: ::